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pCD-Ugi22
(Plasmid #207004)

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Item Catalog # Description Quantity Price (USD)
Plasmid 207004 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pGGC000
  • Backbone manufacturer
    Jan Lohmann Lab
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 3078
  • Vector type
    Golden Gate Compatible cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Utricularia gibba insulator 22
  • Alt name
    Ugi22
  • Species
    Utricularia gibba
  • Insert Size (bp)
    390
  • GenBank ID
    BK063664

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAGGCTTTACACTTTATGCTTCCGGC
  • 3′ sequencing primer AGGGTTTTCCCAGTCACGACGTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD-Ugi22 was a gift from Wayne Parrott (Addgene plasmid # 207004 ; http://n2t.net/addgene:207004 ; RRID:Addgene_207004)

  • For your References section:

    Mining the Utricularia gibba genome for insulator-like elements for genetic engineering. Laspisa D, Llla-Berenguer E, Bang S, Schmitz RJ, Parrott W, Wallace J. Front Plant Sci. 2023 Nov 8;14:1279231. doi: 10.3389/fpls.2023.1279231. eCollection 2023. 10.3389/fpls.2023.1279231 PubMed 38023853
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