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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20700 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4620
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Vector typeMammalian Expression
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Selectable markersG418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDamage-specific DNA binding protein 2
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Alt nameDDB2
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SpeciesH. sapiens (human)
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Insert Size (bp)1284
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GenBank IDNM_000107
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Entrez GeneDDB2 (a.k.a. DDBB, UV-DDB2, XPE)
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Tag
/ Fusion Protein
- myc3 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-myc3-DDB2 was a gift from Yue Xiong (Addgene plasmid # 20700) -
For your References section:
Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage. Hu J, McCall CM, Ohta T, Xiong Y. Nat Cell Biol. 2004 Oct . 6(10):1003-9. 10.1038/ncb1172 PubMed 15448697
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/34/24/b2912fc0-af62-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)