pCAS
(Plasmid #206994)

• Purpose: Plasmid expressing both SpCas9 and SaCas9
• Depositing Lab: Alejandro Chavez
• Publication: Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi: 10.1126/sciadv.adg8771. Epub 2024 May 1.

Backbone

• Vector backbone: pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
• Modifications to backbone: 3xHA to P2A
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpCas9 - P2A - SaCas9
• Alt name: Staphylococcus aureus Cas9
• Alt name: Streptococcus pyogenes Cas9
• Entrez Gene: NEWENTRY
• Entrez Gene: NEWENTRY
• Promoter: CMV
• Tag / Fusion Protein:
  • P2A

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: P2A binding primer

Resource Information

• Supplemental Documents:
  • pCAS.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.11.16.516691v1 for bioRxiv preprint.