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Addgene

pCAS
(Plasmid #206994)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206994 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
  • Modifications to backbone
    3xHA to P2A
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 - P2A - SaCas9
  • Alt name
    Staphylococcus aureus Cas9
  • Alt name
    Streptococcus pyogenes Cas9
  • Entrez Gene
    NEWENTRY
  • Entrez Gene
    NEWENTRY
  • Promoter CMV
  • Tag / Fusion Protein
    • P2A

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAS was a gift from Alejandro Chavez (Addgene plasmid # 206994 ; http://n2t.net/addgene:206994 ; RRID:Addgene_206994)
  • For your References section:

    High-throughput tagging of endogenous loci for rapid characterization of protein function. Kim J, Kratz AF, Chen S, Sheng J, Kim HK, Zhang L, Singh BK, Chavez A. Sci Adv. 2024 May 3;10(18):eadg8771. doi: 10.1126/sciadv.adg8771. Epub 2024 May 1. 10.1126/sciadv.adg8771 PubMed 38691600