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pTRIPZ-NLS-PTEN
(Plasmid #206984)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206984 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIPZ
  • Backbone size w/o insert (bp) 13320
  • Modifications to backbone
    TurboRFP and shRNA cloning site were removed
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PTEN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1238
  • Entrez Gene
    PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
  • Promoter tet-on promoter
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer GTCGAGGTAGGCGTGTACG
  • 3′ sequencing primer TCGTCTGACGTGGCAGCGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIPZ-NLS-PTEN was a gift from Ming Chen (Addgene plasmid # 206984 ; http://n2t.net/addgene:206984 ; RRID:Addgene_206984)

  • For your References section:

    Nuclear PTEN Regulates Thymidylate Biosynthesis in Human Prostate Cancer Cell Lines. Loh ZN, Wang ME, Wan C, Asara JM, Ji Z, Chen M. Metabolites. 2023 Aug 11;13(8):939. doi: 10.3390/metabo13080939. 10.3390/metabo13080939 PubMed 37623882
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