pTRIPZ
(Plasmid
#206981)
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Purpose(Empty Backbone) empty pTRIPZ vector for cloning of doxycycline-inducible gene expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTRIPZ
- Backbone size (bp) 13320
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Modifications to backboneTurboRFP and shRNA cloning site were removed
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- 5′ sequencing primer GTCGAGGTAGGCGTGTACG
- 3′ sequencing primer TCGTCTGACGTGGCAGCGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRIPZ was a gift from Ming Chen (Addgene plasmid # 206981 ; http://n2t.net/addgene:206981 ; RRID:Addgene_206981) -
For your References section:
Nuclear PTEN Regulates Thymidylate Biosynthesis in Human Prostate Cancer Cell Lines. Loh ZN, Wang ME, Wan C, Asara JM, Ji Z, Chen M. Metabolites. 2023 Aug 11;13(8):939. doi: 10.3390/metabo13080939. 10.3390/metabo13080939 PubMed 37623882
Map uploaded by the depositor.
