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Addgene

SpIMPDH
(Plasmid #206980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206980 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28b
  • Backbone manufacturer
    Genscript
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Express protein in One Shot BL21-AI E. Coli
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpIMPDH
  • Alt name
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase
  • Alt name
    EC. 1.1.1.205
  • Species
    Streptococcus pyogenes
  • Entrez Gene
    guaB (a.k.a. DQM35_RS08830, NCTC12064_01736)
  • Tag / Fusion Protein
    • His tag (with TEV cleavage site) (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpIMPDH was a gift from Vern Schramm (Addgene plasmid # 206980 ; http://n2t.net/addgene:206980 ; RRID:Addgene_206980)

  • For your References section:

    Kinetic Characterization and Inhibition of Trypanosoma cruzi Hypoxanthine-Guanine Phosphoribosyltransferases. Glockzin K, Kostomiris D, Minnow YVT, Suthagar K, Clinch K, Gai S, Buckler JN, Schramm VL, Tyler PC, Meek TD, Katzfuss A. Biochemistry. 2022 Oct 4;61(19):2088-2105. doi: 10.1021/acs.biochem.2c00312. Epub 2022 Sep 15. 10.1021/acs.biochem.2c00312 PubMed 36193631
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