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Addgene

pEPSA5Cas9syn2
(Plasmid #206963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEPSA5
  • Backbone size (bp) 5500
  • Modifications to backbone
    removed five motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNTCG, and GWAGNNNNNGAT
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    chloramphenicol resistance in S. aureus
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEPSA5Cas9syn2 was a gift from Daijie Chen (Addgene plasmid # 206963 ; http://n2t.net/addgene:206963 ; RRID:Addgene_206963)

  • For your References section:

    Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit. Yang P, Yang J, Lin T, Liu Q, Yin Y, Chen D, Yang S. ACS Synth Biol. 2023 Oct 13. doi: 10.1021/acssynbio.3c00339. 10.1021/acssynbio.3c00339 PubMed 37830328
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