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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4620
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Vector typeMammalian Expression
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Selectable markersG418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecullin 7
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Alt nameKIAA0076
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Alt nameCUL7
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SpeciesH. sapiens (human)
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Insert Size (bp)5124
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GenBank IDQ14999
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Entrez GeneCUL7 (a.k.a. 3M1, CUL-7, KIAA0076, dJ20C7.5)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-Cul7 was a gift from Yue Xiong (Addgene plasmid # 20696 ; http://n2t.net/addgene:20696 ; RRID:Addgene_20696) -
For your References section:
Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function. Andrews P, He YJ, Xiong Y. Oncogene. 2006 Aug 3. 25(33):4534-48. 10.1038/sj.onc.1209490 PubMed 16547496
Map uploaded by the depositor.
