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Addgene

pET21b-hHNF4aORF-1
(Plasmid #206959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5442
  • Total vector size (bp) 6867
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2)
  • Alt name
    HNF4a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1425
  • Entrez Gene
    HNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TTGCTAGCATGCGACTCTCCAAAACCC
  • 3′ sequencing primer TTGCGGCCGCGATAACTTCCTGCTTGGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21b-hHNF4aORF-1 was a gift from Catharine Ross & Reza Zolfaghari (Addgene plasmid # 206959 ; http://n2t.net/addgene:206959 ; RRID:Addgene_206959)
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