CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG
(Plasmid
#206956)
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PurposeAAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206956 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-CaMKIIa
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet-I, MmMT3, QtEnc
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The AAVs (AAV5) used in our study were produced by UNC Vector Core at Chapel Hill
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG was a gift from Gil Westmeyer (Addgene plasmid # 206956 ; http://n2t.net/addgene:206956 ; RRID:Addgene_206956) -
For your References section:
Genetically encoded barcodes for correlative volume electron microscopy. Sigmund F, Berezin O, Beliakova S, Magerl B, Drawitsch M, Piovesan A, Goncalves F, Bodea SV, Winkler S, Bousraou Z, Grosshauser M, Samara E, Pujol-Marti J, Schadler S, So C, Irsen S, Walch A, Kofler F, Piraud M, Kornfeld J, Briggman K, Westmeyer GG. Nat Biotechnol. 2023 Apr 17. doi: 10.1038/s41587-023-01713-y. 10.1038/s41587-023-01713-y PubMed 37069313