1M-Qt-antiGFP
(Plasmid
#206951)
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PurposeMammalian expression of 1M-Qt EMcapsulins targeting GFP
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 3.1 (+) Zeo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMmMT3, QtEnc, antiGFP-intrabody
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Due to the 100% surface decoration with the anti-GFP binder, this construct is likely not suitable for targeting of endogenous proteins
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
1M-Qt-antiGFP was a gift from Gil Westmeyer (Addgene plasmid # 206951 ; http://n2t.net/addgene:206951 ; RRID:Addgene_206951) -
For your References section:
Genetically encoded barcodes for correlative volume electron microscopy. Sigmund F, Berezin O, Beliakova S, Magerl B, Drawitsch M, Piovesan A, Goncalves F, Bodea SV, Winkler S, Bousraou Z, Grosshauser M, Samara E, Pujol-Marti J, Schadler S, So C, Irsen S, Walch A, Kofler F, Piraud M, Kornfeld J, Briggman K, Westmeyer GG. Nat Biotechnol. 2023 Apr 17. doi: 10.1038/s41587-023-01713-y. 10.1038/s41587-023-01713-y PubMed 37069313