pcDNA3-myc3-Cul7
(Plasmid #20695)

• Depositing Lab: Yue Xiong
• Publication: Andrews et al Oncogene. 2006 Aug 3. 25(33):4534-48.

Backbone

• Vector backbone: pcDNA3-myc3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5486
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cullin 7
• Alt name: KIAA0076
• Alt name: CUL7
• Species: H. sapiens (human)
• Insert Size (bp): 5124
• GenBank ID: Q14999
• Entrez Gene: CUL7 (a.k.a. 3M1, CUL-7, KIAA0076, dJ20C7.5)
• Tag / Fusion Protein:
  • Myc3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI/ XhoI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry