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Addgene

sfGFP-SasG(5G)-mCherry
(Plasmid #206947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206947 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.1 (+) Zeo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP-SaSasG (5G)-mCherry
  • Species
    A.victoria, S.aureus, Discosoma sp
  • Tags / Fusion Proteins
    • sfGFP (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sfGFP-SasG(5G)-mCherry was a gift from Gil Westmeyer (Addgene plasmid # 206947 ; http://n2t.net/addgene:206947 ; RRID:Addgene_206947)

  • For your References section:

    Genetically encoded barcodes for correlative volume electron microscopy. Sigmund F, Berezin O, Beliakova S, Magerl B, Drawitsch M, Piovesan A, Goncalves F, Bodea SV, Winkler S, Bousraou Z, Grosshauser M, Samara E, Pujol-Marti J, Schadler S, So C, Irsen S, Walch A, Kofler F, Piraud M, Kornfeld J, Briggman K, Westmeyer GG. Nat Biotechnol. 2023 Apr 17. doi: 10.1038/s41587-023-01713-y. 10.1038/s41587-023-01713-y PubMed 37069313
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