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Addgene

pGEX6p1-hPdcd4
(Plasmid #20693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-1
  • Backbone manufacturer
    GE
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a, 37 degrees
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Programmed Cell Death Protein 4
  • Alt name
    Pdcd4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1410
  • Mutation
    I36V- does not alter function
  • GenBank ID
    NM_014456
  • Entrez Gene
    PDCD4 (a.k.a. H731)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX6p1-hPdcd4 was a gift from Haiwei Song (Addgene plasmid # 20693 ; http://n2t.net/addgene:20693 ; RRID:Addgene_20693)

  • For your References section:

    Structural basis for translational inhibition by the tumour suppressor Pdcd4. Loh PG, Yang HS, Walsh MA, Wang Q, Wang X, Cheng Z, Liu D, Song H. EMBO J. 2009 Feb 4. 28(3):274-85. 10.1038/emboj.2008.278 PubMed 19153607
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