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pAAV INS2x mRFP-3MREs (AAV-beta)
(Plasmid #206928)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV INS2x-3MREs
  • Backbone manufacturer
    Sunghee Chai, Markus Grompe
  • Backbone size w/o insert (bp) 4881
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mRFP
  • Species
    Synthetic
  • Insert Size (bp)
    678
  • Promoter INSx2

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The combination of human insulin promoter (INSx2) and 3MREs (microRNA recognition elements) enables transgene expression in beta, and not in alpha cells, in pancreatic islets of human and mouse.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV INS2x mRFP-3MREs (AAV-beta) was a gift from Markus Grompe (Addgene plasmid # 206928 ; http://n2t.net/addgene:206928 ; RRID:Addgene_206928)

  • For your References section:

    Development of a Beta Cell-Specific Expression Control Element for Recombinant Adeno-Associated Virus. Chai S, Kim Y, Galivo F, Dorrell C, Wakefield L, Posey J, Ackermann AM, Kaestner KH, Hebrok M, Grompe M. Hum Gene Ther. 2022 Aug;33(15-16):789-800. doi: 10.1089/hum.2021.219. Epub 2022 May 10. 10.1089/hum.2021.219 PubMed 35297680
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