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pAAV MP-135 mRFP
(Plasmid #206927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV MP-135
  • Backbone manufacturer
    Sunghee Chai, Markus Grompe
  • Backbone size w/o insert (bp) 4247
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mRFP
  • Species
    Synthetic
  • Insert Size (bp)
    678
  • Promoter MP-135

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site NsiI (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Intron and WPRE are cloned to enhance expression of transgene and as a stuffer DNA. These elements are not required for the promoter activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV MP-135 mRFP was a gift from Markus Grompe (Addgene plasmid # 206927 ; http://n2t.net/addgene:206927 ; RRID:Addgene_206927)

  • For your References section:

    Strong ubiquitous micro-promoters for recombinant adeno-associated viral vectors. Chai S, Wakefield L, Norgard M, Li B, Enicks D, Marks DL, Grompe M. Mol Ther Methods Clin Dev. 2023 May 17;29:504-512. doi: 10.1016/j.omtm.2023.05.013. eCollection 2023 Jun 8. 10.1016/j.omtm.2023.05.013 PubMed 37287749
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