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Addgene

pEMBL-Si-EGFP
(Plasmid #20692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEMBL
  • Backbone size w/o insert (bp) 5770
  • Vector type
    Mammalian Expression ; Adeno associated virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6-siEGFP
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMBL-Si-EGFP was a gift from Tom Misteli (Addgene plasmid # 20692 ; http://n2t.net/addgene:20692 ; RRID:Addgene_20692)

  • For your References section:

    Lamin A/C-mediated neuromuscular junction defects in Emery-Dreifuss muscular dystrophy. Mejat A, Decostre V, Li J, Renou L, Kesari A, Hantaï D, Stewart CL, Xiao X, Hoffman E, Bonne G, Misteli T. J Cell Biol. 2009 Jan 12. 184(1):31-44. 10.1083/jcb.200811035 PubMed 19124654
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