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pSA351_SCP1_intron_eGFP
(Plasmid #206906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCCL-PGK
  • Total vector size (bp) 7552
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    717
  • Promoter SCP1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACTAGCCTCGAGTCGCTGAGCTCCCTAGGGTAC
  • 3′ sequencing primer TGGTGGCGACCGGTGACCTGGGAGTGGACAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSA351_SCP1_intron_eGFP was a gift from Stein Aerts (Addgene plasmid # 206906 ; http://n2t.net/addgene:206906 ; RRID:Addgene_206906)

  • For your References section:

    Cell type directed design of synthetic enhancers. Taskiran II, Spanier KI, Dickmanken H, Kempynck N, Pancikova A, Eksi EC, Hulselmans G, Ismail JN, Theunis K, Vandepoel R, Christiaens V, Mauduit D, Aerts S. Nature. 2023 Dec 12. doi: 10.1038/s41586-023-06936-2. 10.1038/s41586-023-06936-2 PubMed 38086419
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