pU6-sgRNA-CBh-eGFP
(Plasmid
#206885)
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Purpose(Empty Backbone) cloning backbone for sgRNA using OptScf2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepU6-sgRNA-OptScf2
- Backbone size (bp) 4967
- Promoter U6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gagggcctatttcccatga (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU6-sgRNA-CBh-eGFP was a gift from Jacob Giehm Mikkelsen (Addgene plasmid # 206885 ; http://n2t.net/addgene:206885 ; RRID:Addgene_206885) -
For your References section:
Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification. Haldrup J, Andersen S, Labial ARL, Wolff JH, Frandsen FP, Skov TW, Rovsing AB, Nielsen I, Jakobsen TS, Askou AL, Thomsen MK, Corydon TJ, Thomsen EA, Mikkelsen JG. Nucleic Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676. 10.1093/nar/gkad676 PubMed 37678882
Map uploaded by the depositor.
