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Addgene

pLVNP2.0-SpCas9
(Plasmid #206880)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206880 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVNP2.0
  • Total vector size (bp) 14588
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • Insert Size (bp)
    4101
  • Entrez Gene
    cas9 (a.k.a. SD90_RS03825, SD90_03830)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVNP2.0-SpCas9 was a gift from Jacob Giehm Mikkelsen (Addgene plasmid # 206880 ; http://n2t.net/addgene:206880 ; RRID:Addgene_206880)

  • For your References section:

    Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification. Haldrup J, Andersen S, Labial ARL, Wolff JH, Frandsen FP, Skov TW, Rovsing AB, Nielsen I, Jakobsen TS, Askou AL, Thomsen MK, Corydon TJ, Thomsen EA, Mikkelsen JG. Nucleic Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676. 10.1093/nar/gkad676 PubMed 37678882
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