pET-tTALE-mTurquoise2-15b
(Plasmid
#206871)
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PurposeExpress tTALE-mTurquoise2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206871 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5625
- Total vector size (bp) 1776
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name7-mer tTALE-mTurquoise2
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Insert Size (bp)1776
- Promoter T7 promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-tTALE-mTurquoise2-15b was a gift from Kyubong Jo (Addgene plasmid # 206871 ; http://n2t.net/addgene:206871 ; RRID:Addgene_206871) -
For your References section:
21 Fluorescent Protein-Based DNA Staining Dyes. Kim YT, Oh H, Seo MJ, Lee DH, Shin J, Bong S, Heo S, Hapsari ND, Jo K. Molecules. 2022 Aug 17;27(16):5248. doi: 10.3390/molecules27165248. 10.3390/molecules27165248 PubMed 36014487