pBS-AMV-δ1L9'S-nAchR
(Plasmid
#20687)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenicotinic acetylcholine receptors
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Alt namenAchR
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Alt nameDelta1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1500
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MutationLeucine at 9' position in M2 region changed to Serine OR (L265S)
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GenBank IDBC41387
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Tags
/ Fusion Proteins
- AMV (N terminal on insert)
- pA 50 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bcl I (unknown if destroyed)
- 3′ cloning site Xba I (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-AMV-δ1L9'S-nAchR was a gift from Henry Lester (Addgene plasmid # 20687 ; http://n2t.net/addgene:20687 ; RRID:Addgene_20687) -
For your References section:
Channel gating governed symmetrically by conserved leucine residues in the M2 domain of nicotinic receptors. Labarca C, Nowak MW, Zhang H, Tang L, Deshpande P, Lester HA. Nature. 1995 Aug 10. 376(6540):514-6. 10.1038/376514a0 PubMed 7637783