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Addgene

pBABE-LynG
(Plasmid #206867)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206867 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro (Plasmid #1764)
  • Backbone manufacturer
    addgene
  • Backbone size w/o insert (bp) 5086
  • Total vector size (bp) 5954
  • Modifications to backbone
    LynG insert
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    This plasmid has been stored as a glycerol stock in NEB stable cells to reduce recombination, however the Herten Lab uses DH5alpha without issue.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LynG
  • Alt name
    LynAnchor-eGFP
  • Species
    M. musculus (mouse); Aequorea victoria
  • Insert Size (bp)
    774
  • Mutation
    Contains Lyn amino acids 1-16
  • GenBank ID
    NM_001111096.2 AAB02572.1
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-LynG was a gift from Dirk-Peter Herten (Addgene plasmid # 206867 ; http://n2t.net/addgene:206867 ; RRID:Addgene_206867)

  • For your References section:

    ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting. Tashev SA, Euchner J, Yserentant K, Hanselmann S, Hild F, Chmielewicz W, Hummert J, Schworer F, Tsopoulidis N, Germer S, Sassmannshausen Z, Fackler OT, Klingmuller U, Herten DP. Nat Methods. 2024 Sep;21(9):1708-1715. doi: 10.1038/s41592-024-02376-6. Epub 2024 Aug 8. 10.1038/s41592-024-02376-6 PubMed 39117875
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