pBABE-ProDOL
(Plasmid #206866)

• Purpose: Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag
• Depositing Lab: Dirk-Peter Herten
• Publication: Tashev et al Nat Methods (2024)

Backbone

• Vector backbone: pBABE-puro (Plasmid #1764)
• Backbone manufacturer: addgene
• Backbone size w/o insert (bp): 5086
• Total vector size (bp): 7474
• Modifications to backbone: ProDOL insert
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: This plasmid has been stored as a glycerol stock in NEB stable cells to reduce recombination, however the Herten Lab uses DH5alpha without issue.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ProDOL
• Alt name: LynAnchor-eGFP-SNAP-tag-HaloTag
• Alt name: gSEP
• Species: H. sapiens (human), M. musculus (mouse); Aequorea victoria, Rhodococcus rhodochrous
• Insert Size (bp): 774
• Mutation: Contains Lyn amino acids 1-16
• GenBank ID: NM_001111096.2 AAB02572.1
• Tags / Fusion Proteins:
  • eGFP (C terminal on insert)
  • SNAP-tag (C terminal on insert)
  • HaloTag (C terminal on insert)
  • His-Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pBABE-ProDOL-sequence.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry