pBABE-ProDOL
(Plasmid
#206866)
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PurposeExpression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABE-puro (Plasmid #1764)
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Backbone manufactureraddgene
- Backbone size w/o insert (bp) 5086
- Total vector size (bp) 7474
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Modifications to backboneProDOL insert
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsThis plasmid has been stored as a glycerol stock in NEB stable cells to reduce recombination, however the Herten Lab uses DH5alpha without issue.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProDOL
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Alt nameLynAnchor-eGFP-SNAP-tag-HaloTag
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Alt namegSEP
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SpeciesH. sapiens (human), M. musculus (mouse); Aequorea victoria, Rhodococcus rhodochrous
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Insert Size (bp)774
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MutationContains Lyn amino acids 1-16
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GenBank IDNM_001111096.2 AAB02572.1
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Tags
/ Fusion Proteins
- eGFP (C terminal on insert)
- SNAP-tag (C terminal on insert)
- HaloTag (C terminal on insert)
- His-Tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-ProDOL was a gift from Dirk-Peter Herten (Addgene plasmid # 206866 ; http://n2t.net/addgene:206866 ; RRID:Addgene_206866) -
For your References section:
ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting. Tashev SA, Euchner J, Yserentant K, Hanselmann S, Hild F, Chmielewicz W, Hummert J, Schworer F, Tsopoulidis N, Germer S, Sassmannshausen Z, Fackler OT, Klingmuller U, Herten DP. Nat Methods. 2024 Sep;21(9):1708-1715. doi: 10.1038/s41592-024-02376-6. Epub 2024 Aug 8. 10.1038/s41592-024-02376-6 PubMed 39117875