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Addgene

M13-kmR
(Plasmid #206861)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    M13KE
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 7222
  • Total vector size (bp) 8048
  • Modifications to backbone
    NotI restriction site added to M13 gene III
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Kanamycin resistance gene
  • Alt name
    M13KE-NotI-Kan
  • Insert Size (bp)
    816
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

R179W and S299L mutations in M13 gene II do not affect plasmid replication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M13-kmR was a gift from Irene Chen (Addgene plasmid # 206861 ; http://n2t.net/addgene:206861 ; RRID:Addgene_206861)

  • For your References section:

    Inhibition of bacterial conjugation by phage M13 and its protein g3p: quantitative analysis and model. Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA. PLoS One. 2011;6(5):e19991. doi: 10.1371/journal.pone.0019991. Epub 2011 May 26. 10.1371/journal.pone.0019991 PubMed 21637841
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