M13-kmR
(Plasmid #206861)

• Purpose: M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)
• Depositing Lab: Irene Chen
• Publication: Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371/journal.pone.0019991. Epub 2011 May 26.

Backbone

• Vector backbone: M13KE
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 7222
• Total vector size (bp): 8048
• Modifications to backbone: NotI restriction site added to M13 gene III
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Kanamycin resistance gene
• Alt name: M13KE-NotI-Kan
• Insert Size (bp): 816
• Promoter: lac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • M13-kmR.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

R179W and S299L mutations in M13 gene II do not affect plasmid replication.