Skip to main content
Addgene

pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA
(Plasmid #206860)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEMBL8
  • Vector type
    AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mLdlr gRNA
  • gRNA/shRNA sequence
    GGGcAGGCGCAGGTGAATTTGG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ldlr (a.k.a. Hlb301)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The gRNA targeting Ldlr was originally published in PMID 30026278 and is also included in Addgene plasmid #109311. We have moved this gRNA to the Addgene plasmid #109314 to generate this one, which has hepatocyte-specific expression of SaCas9

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA was a gift from William Lagor (Addgene plasmid # 206860 ; http://n2t.net/addgene:206860 ; RRID:Addgene_206860)

  • For your References section:

    In Vivo Gene Editing in Lipid and Atherosclerosis Research. De Giorgi M, Jarrett KE, de Aguiar Vallim TQ, Lagor WR. Methods Mol Biol. 2022;2419:673-713. doi: 10.1007/978-1-0716-1924-7_42. 10.1007/978-1-0716-1924-7_42 PubMed 35237996
Related items: