pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA
(Plasmid
#206860)
-
PurposeAn AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEMBL8
-
Vector typeAAV, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namemLdlr gRNA
-
gRNA/shRNA sequenceGGGcAGGCGCAGGTGAATTTGG
-
SpeciesM. musculus (mouse)
-
Entrez GeneLdlr (a.k.a. Hlb301)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The gRNA targeting Ldlr was originally published in PMID 30026278 and is also included in Addgene plasmid #109311. We have moved this gRNA to the Addgene plasmid #109314 to generate this one, which has hepatocyte-specific expression of SaCas9
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA was a gift from William Lagor (Addgene plasmid # 206860 ; http://n2t.net/addgene:206860 ; RRID:Addgene_206860)
Map uploaded by the depositor.
