pPt_Sap
(Plasmid #206856)

• Purpose: (Empty Backbone) A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.
• Depositing Lab: Bogumil Karas
• Publication: Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.

Backbone

• Vector backbone: pINTO_7/8 (pCC1BAC-based)
• Backbone size (bp): 132896
• Modifications to backbone: The entire P. tricornutum chloroplast genome (117 kb) was assembled and cloned using the pINTO_7/8 vector. The cloning vector and genome were domesticated for SapI; however, an endogenous SapI site is still present in the integrated URA3 marker.
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: URA3, HIS3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EPI300
• Growth instructions: The backbone contains an OriV. It can be induced to high-copy replication in the presence of arabinose if maintained in the EPI300 strain (or any other E. coli strain with the pBAD/trfA system).
• Copy number: High Copy

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid is derived from pDMI-2, which is a pCC1BAC-based plasmid that was originally engineered by Designer Microbes Inc (London, Ontario).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543740v1 for bioRxiv preprint.

Addgene could not confirm the entire plasmid due to difficulty with sequencing.