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Addgene

pBS-AMV-β1L9'S-nAchR
(Plasmid #20685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nicotinic acetylcholine receptors
  • Alt name
    nAchR
  • Alt name
    Beta1
  • Alt name
    Chrnb1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2100
  • Mutation
    Leucine at 9' position in M2 region changed to Serine OR (L262S)
  • GenBank ID
    BC023318
  • Entrez Gene
    Chrnb1 (a.k.a. Achr-2, Acrb)
  • Tags / Fusion Proteins
    • AMV (N terminal on insert)
    • pA 50 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bst E II (unknown if destroyed)
  • 3′ cloning site BstX I (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-AMV-β1L9'S-nAchR was a gift from Henry Lester (Addgene plasmid # 20685 ; http://n2t.net/addgene:20685 ; RRID:Addgene_20685)

  • For your References section:

    Channel gating governed symmetrically by conserved leucine residues in the M2 domain of nicotinic receptors. Labarca C, Nowak MW, Zhang H, Tang L, Deshpande P, Lester HA. Nature. 1995 Aug 10. 376(6540):514-6. 10.1038/376514a0 PubMed 7637783
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