pCHAP3_URA
(Plasmid #206848)

• Purpose: (Empty Backbone) A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.
• Depositing Lab: Bogumil Karas
• Publication: Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670.

Backbone

• Vector backbone: pSAP (pCC1BAC-based)
• Backbone size (bp): 27019
• Modifications to backbone: A region of the P. tricornutum chloroplast genome (designated fragment 3) was assembled and cloned using the pSAP backbone. A second yeast selection marker for URA3 was integrated into a non-coding region of chloroplast fragment 3. The URA3 marker contains an endogenous SapI site.
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: TRP1, URA3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): EPI300
• Growth instructions: The backbone contains an OriV sequence. This means that the plasmid can be induced to high-copy replication in the presence of arabinose if maintained in the EPI300 strain (or any other E. coli strain harbouring the pBAD/trfA system).
• Copy number: High Copy

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The pSAP cloning vector is derived from pCCBAC1_LC_TRP (GenBank accession number MN982904)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543740v1 for bioRxiv preprint.