pGL4.23-5xE-box
(Plasmid
#206841)
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PurposeThe plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.23[luc2/minP]
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4283
- Total vector size (bp) 4595
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name5x E-box ((GCCACGTGCA)
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SpeciesH. sapiens (human)
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Insert Size (bp)312
Resource Information
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A portion of this plasmid was derived from a plasmid made byAZENTA Life Sciences, GENEWIZ Germany.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.23-5xE-box was a gift from Tassula Proikas-Cezanne (Addgene plasmid # 206841 ; http://n2t.net/addgene:206841 ; RRID:Addgene_206841) -
For your References section:
The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan extension. Sporbeck K, Haas ML, Pastor-Maldonado CJ, Schussele DS, Hunter C, Takacs Z, Diogo de Oliveira AL, Franz-Wachtel M, Charsou C, Pfisterer SG, Gubas A, Haller PK, Knorr RL, Kaulich M, Macek B, Eskelinen EL, Simonsen A, Proikas-Cezanne T. Commun Biol. 2023 Aug 24;6(1):872. doi: 10.1038/s42003-023-05236-9. 10.1038/s42003-023-05236-9 PubMed 37620393