pHJ425
(Plasmid #20683)

• Depositing Lab: Stephen Shaw
• Publication: Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 1758
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a, Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ezrin
• Alt name: EZR
• Species: H. sapiens (human)
• Insert Size (bp): 1758
• Mutation: Changed Lys 253, 254, 262, 263 to Asn; and changed thr 567 to Asp.
• GenBank ID: NM_003379
• Entrez Gene: EZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GTGCACAAGTCTGGGTAC
• 3′ sequencing primer: GTGAGCTACCATGTCCAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry