pHJ424
(Plasmid
#20682)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 1758
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEzrin
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Alt nameEZR
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SpeciesH. sapiens (human)
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Insert Size (bp)1758
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MutationChanged Lys 253, 254, 262, 263 to Asn
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GenBank IDNM_003379
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Entrez GeneEZR (a.k.a. CVIL, CVL, HEL-S-105, VIL2)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer GTGCACAAGTCTGGGTAC
- 3′ sequencing primer GTGAGCTACCATGTCCAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHJ424 was a gift from Stephen Shaw (Addgene plasmid # 20682 ; http://n2t.net/addgene:20682 ; RRID:Addgene_20682) -
For your References section:
Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane. Hao JJ, Liu Y, Kruhlak M, Debell KE, Rellahan BL, Shaw S. J Cell Biol. 2009 Feb 9. 184(3):451-62. 10.1083/jcb.200807047 PubMed 19204146