lentiCRISPRv2-Blast-mU6
(Plasmid #206806)

• Purpose: (Empty Backbone) pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette
• Depositing Lab: Jolanda Van Leeuwen
• Publication: Global analysis of suppressor mutations that rescue human genetic defects. (unpublished)

Backbone

• Vector backbone: lentiCRISPR v2-Blast
• Backbone manufacturer: Mohan Babu
• Backbone size (bp): 14684
• Modifications to backbone: Amplified mU6 promoter from plasmid pmU6-gRNA (Addgene Plasmid #53187) and replaced the hU6 promoter in lentiCRISPRv2-Blast (Addgene Plasmid #83480) with mU6 promoter by restriction-ligation. Verified by Sanger sequencing.
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Promoter: mU6
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • plenticrisprv2-blast-mu6.gb
• A portion of this plasmid was derived from a plasmid made by: The mU6 promoter was amplified from a plasmid obtained from Addgene (Plasmid #53187). The backbone was purchased from Addgene (Plasmid #83480).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry