pTrc_AraC-S170-LOV
(Plasmid
#206805)
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PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206805 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTKEI-Dest modified
- Backbone size w/o insert (bp) 3656
- Total vector size (bp) 4961
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAraC-AsLOV2 fusion
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SpeciesE. coli, A. sativa
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Insert Size (bp)1305
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Tag
/ Fusion Protein
- His-tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrc_AraC-S170-LOV was a gift from Dominik Niopek (Addgene plasmid # 206805 ; http://n2t.net/addgene:206805 ; RRID:Addgene_206805) -
For your References section:
Dissecting the Determinants of Domain Insertion Tolerance and Allostery in Proteins. Mathony J, Aschenbrenner S, Becker P, Niopek D. Adv Sci (Weinh). 2023 Oct;10(28):e2303496. doi: 10.1002/advs.202303496. Epub 2023 Aug 10. 10.1002/advs.202303496 PubMed 37562980