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Addgene

pHJ386
(Plasmid #20679)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Moesin
  • Alt name
    MSN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1731
  • Mutation
    Changed Lys 253, 254, 262, 263 to Asn; Changed Thr 558 to Asp
  • GenBank ID
    NM_002444
  • Entrez Gene
    MSN (a.k.a. HEL70, IMD50)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GTGCATAAGTCTGGCTAC
  • 3′ sequencing primer GATGAGCAGGATGAGAAT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHJ386 was a gift from Stephen Shaw (Addgene plasmid # 20679 ; http://n2t.net/addgene:20679 ; RRID:Addgene_20679)

  • For your References section:

    Phospholipase C-mediated hydrolysis of PIP2 releases ERM proteins from lymphocyte membrane. Hao JJ, Liu Y, Kruhlak M, Debell KE, Rellahan BL, Shaw S. J Cell Biol. 2009 Feb 9. 184(3):451-62. 10.1083/jcb.200807047 PubMed 19204146
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