pHJ322
(Plasmid #20677)

• Depositing Lab: Stephen Shaw
• Publication: Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Moesin
• Alt name: MSN
• Species: H. sapiens (human)
• Insert Size (bp): 1731
• Mutation: Changed Thr 558 to Asp
• GenBank ID: NM_002444
• Entrez Gene: MSN (a.k.a. HEL70, IMD50)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GTGCATAAGTCTGGCTAC
• 3′ sequencing primer: GATGAGCAGGATGAGAAT

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry