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Addgene

Lenti-BAT-RED
(Plasmid #20674)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript-BATgal
  • Backbone size w/o insert (bp) 7146
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wnt reporter dsRED
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13for20
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

History:

1)From BAT-gal vector (S. Piccolo lab), cut with HindIII+BamHI to remove lacZ sequence including the nuclear sequence and the polyA sequence.

2) from DsRED.T4 (B. Glick lab), PCR-amplified DsRED + SV40 polyA with primers that contain the predicted NLS sequence from BATgal (see attachments)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-BAT-RED was a gift from Tannishtha Reya (Addgene plasmid # 20674 ; http://n2t.net/addgene:20674 ; RRID:Addgene_20674)
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