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Addgene

MSCV.PCAT.IRES.GFP
(Plasmid #20673)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20673 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6573
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-catenin
  • Species
    unknown
  • Mutation
    delta GSK-KT3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (destroyed during cloning)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer pBabe-5
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Reya lab has asked that Addgene take this plasmid offline because it is the same as Plasmid #14717. Please go to www.addgene.org/14717 to order this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV.PCAT.IRES.GFP was a gift from Tannishtha Reya (Addgene plasmid # 20673)
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