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Addgene

pCMV-miR30
(Plasmid #20670)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20670 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-PL
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miR-30
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    93
  • Entrez Gene
    MIR30A (a.k.a. MIRN30A, mir-30a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV promoter primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see https://pubmed.ncbi.nlm.nih.gov/12554881/ for additional details about this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-miR30 was a gift from Bryan Cullen (Addgene plasmid # 20670 ; http://n2t.net/addgene:20670 ; RRID:Addgene_20670)

  • For your References section:

    Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells. Zeng Y, Wagner EJ, Cullen BR. Mol Cell. 2002 Jun . 9(6):1327-33. 10.1016/S1097-2765(02)00541-5 PubMed 12086629
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