pFPV25.1
(Plasmid #20668)

• Purpose: (Empty Backbone) S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.
• Depositing Lab: Raphael Valdivia
• Publication: Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

Backbone

• Vector backbone: pFPV
• Backbone size (bp): 4764
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • GFP mut3 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GFP gene is GFP mut3a from Cormack et al (Gene 173:33-38) and contains engineered ribosome binding site.

DNA inserts can be introduced at any of the polylinker sites upstream of the XbaI site.

Promoter activity can be monitored by measuring GFP synthesis by fluorescence microscopy, fluorimetry or flow cytometry.