HA Display Part 1-4 DO
(Plasmid
#206514)
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Purpose(Empty Backbone) CcdB Dropout vector for parts 1-4 of Hemagglutinin surface expression in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT/TO/intron
- Backbone size (bp) 6738
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Vector typeMammalian Expression
- Promoter CMV promoter
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Tag
/ Fusion Protein
- 3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B TM (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA Display Part 1-4 DO was a gift from IIya Finkelstein (Addgene plasmid # 206514 ; http://n2t.net/addgene:206514 ; RRID:Addgene_206514)
Map uploaded by the depositor.
