Strep-TGT-His
(Plasmid
#206495)
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PurposeExpresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206495 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbone(T7)-2 Vector
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 5874
- Total vector size (bp) 7044
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE. coli tRNA Guanine Transglycosylase
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Alt nameE. coli TGT
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SpeciesE. coli
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Insert Size (bp)1170
- Promoter T7
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Tags
/ Fusion Proteins
- Strep (N terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 PROMOTER
- 3′ sequencing primer T7 TERMINATOR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Strep-TGT-His was a gift from Neal Devaraj (Addgene plasmid # 206495 ; http://n2t.net/addgene:206495 ; RRID:Addgene_206495) -
For your References section:
Site-Specific Covalent Labeling of DNA Substrates by an RNA Transglycosylase. Tota EM, Devaraj NK. J Am Chem Soc. 2023 Apr 12;145(14):8099-8106. doi: 10.1021/jacs.3c00861. Epub 2023 Mar 29. 10.1021/jacs.3c00861 PubMed 36988146