Mito-SNAP
(Plasmid #206480)

• Purpose: Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression
• Depositing Lab: Erika Holzbaur
• Publication: Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005.

Backbone

• Vector backbone: mito-DsRed2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3984
• Total vector size (bp): 633
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
• Species: H. sapiens (human)
• Insert Size (bp): 633
• GenBank ID: NM_004074.3 NM_002412.5
• Promoter: CMV
• Tag / Fusion Protein:
  • SNAPf (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pEGFP-C REV2: 5'-CTTTATTTGTAACCATTATAAGCTGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.06.21.496850 for bioRxiv preprint.