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Addgene

pAc5.1-NOT1 isoform C-mCherry-PH (EB5)
(Plasmid #206474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EB03-pAc5.1 FspAI mcherry PH
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NOT1
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Not1 (a.k.a. Dmel_CG34407, CG1874, CG1884, CG34407, Dmel\CG34407, Dmel_CG1874, Dmel_CG1884, NOT, NOT1, anon-EST:Liang-1.29, anon-WO0118547.221, clone 1.29, not1)
  • Tag / Fusion Protein
    • mcherry PH (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1-NOT1 isoform C-mCherry-PH (EB5) was a gift from Mandy Jeske (Addgene plasmid # 206474 ; http://n2t.net/addgene:206474 ; RRID:Addgene_206474)

  • For your References section:

    ReLo is a simple and quick colocalization assay to identify and characterize direct protein-protein interactions. Salgania HK, Metz J, Jeske M. bioRxiv 2022.03.04.482790 10.1101/2022.03.04.482790
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