pAc5.1-PH-mCherry-Bruno isoform A (HK72)
(Plasmid
#206471)
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PurposeBruno expression in Schneider cells for imaging
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneHK049-pAc5.1 PH mcherry FspAI
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBruno
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SpeciesD. melanogaster (fly)
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Entrez Genearet (a.k.a. Dmel_CG31762, ARET, Aret, BcDNA:GM15173, Bru, Bruno, CG31762, CG6319, Dm Bru, Dmel\CG31762, NEST:bs01g04, NEST:bs34h11, aret/bruno1, arrest/Bruno, bru, bruno, ms(2)01284)
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Tag
/ Fusion Protein
- PH mcherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.03.04.482790v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1-PH-mCherry-Bruno isoform A (HK72) was a gift from Mandy Jeske (Addgene plasmid # 206471 ; http://n2t.net/addgene:206471 ; RRID:Addgene_206471) -
For your References section:
ReLo is a simple and quick colocalization assay to identify and characterize direct protein-protein interactions. Salgania HK, Metz J, Jeske M. bioRxiv 2022.03.04.482790 10.1101/2022.03.04.482790