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pAc5.1-mEGFP-human FRB (HK168)
(Plasmid #206443)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206443 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T6-pAc5.1 mEGFP-EcoRV
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FRB
  • Species
    H. sapiens (human)
  • Entrez Gene
    MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1-mEGFP-human FRB (HK168) was a gift from Mandy Jeske (Addgene plasmid # 206443 ; http://n2t.net/addgene:206443 ; RRID:Addgene_206443)

  • For your References section:

    ReLo is a simple and quick colocalization assay to identify and characterize direct protein-protein interactions. Salgania HK, Metz J, Jeske M. bioRxiv 2022.03.04.482790 10.1101/2022.03.04.482790
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