pSAP
(Plasmid
#206429)
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Purpose(Empty Backbone) A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSAP (pCC1BAC-based)
- Backbone size (bp) 9990
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Modifications to backboneDomesticated for SapI
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Growth instructionsThe backbone contains an OriV. This means it can be induced to high-copy replication in the presence of arabinose when the plasmid is maintained in EPI300 cells.
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Copy numberHigh Copy
Resource Information
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A portion of this plasmid was derived from a plasmid made bypCC1Bac-LC-YACTRP (GenBank accession number MN982904).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.06.05.543740v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSAP was a gift from Bogumil Karas (Addgene plasmid # 206429 ; http://n2t.net/addgene:206429 ; RRID:Addgene_206429) -
For your References section:
Design and assembly of the 117-kb Phaeodactylum tricornutum chloroplast genome. Walker EJL, Pampuch M, Chang N, Cochrane RR, Karas BJ. Plant Physiol. 2023 Dec 19:kiad670. doi: 10.1093/plphys/kiad670. 10.1093/plphys/kiad670 PubMed 38114089
Map uploaded by the depositor.
