pcDNA4-Xpress-GBF1(E794K)
(Plasmid
#206418)
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PurposeMammalian expression of hGBF1(E794K)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206418 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4HisMax
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGBF1
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SpeciesH. sapiens (human)
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MutationE794K
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Entrez GeneGBF1 (a.k.a. ARF1GEF, CMT2GG, CMTDI2, CMTDIA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An N-terminal missense mutation.
C158R mutation in GBF1 will not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4-Xpress-GBF1(E794K) was a gift from Kazuhisa Nakayama (Addgene plasmid # 206418 ; http://n2t.net/addgene:206418 ; RRID:Addgene_206418) -
For your References section:
GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Kawamoto K, Yoshida Y, Tamaki H, Torii S, Shinotsuka C, Yamashina S, Nakayama K. Traffic. 2002 Jul;3(7):483-95. doi: 10.1034/j.1600-0854.2002.30705.x. 10.1034/j.1600-0854.2002.30705.x PubMed 12047556