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Addgene

pcDNA3-HA-GBF1(E794K)
(Plasmid #206416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBF1
  • Species
    H. sapiens (human)
  • Mutation
    E794K
  • Entrez Gene
    GBF1 (a.k.a. ARF1GEF, CMT2GG, CMTDI2, CMTDIA)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An N-terminal missense mutation.
C158R mutation in GBF1 will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-GBF1(E794K) was a gift from Kazuhisa Nakayama (Addgene plasmid # 206416 ; http://n2t.net/addgene:206416 ; RRID:Addgene_206416)

  • For your References section:

    GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Kawamoto K, Yoshida Y, Tamaki H, Torii S, Shinotsuka C, Yamashina S, Nakayama K. Traffic. 2002 Jul;3(7):483-95. doi: 10.1034/j.1600-0854.2002.30705.x. 10.1034/j.1600-0854.2002.30705.x PubMed 12047556
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