pEGFP-C1-hEFA6A
(Plasmid
#206412)
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PurposeMammalian expression of hEFA6A
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206412 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEFA6A
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SpeciesH. sapiens (human)
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Entrez GenePSD (a.k.a. EFA6, EFA6A, PSD1, TYL)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-hEFA6A was a gift from Kazuhisa Nakayama & Hye-Won Shin (Addgene plasmid # 206412 ; http://n2t.net/addgene:206412 ; RRID:Addgene_206412) -
For your References section:
EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis. Ueda T, Hanai A, Takei T, Kubo K, Ohgi M, Sakagami H, Takahashi S, Shin HW, Nakayama K. FEBS Lett. 2013 Jun 5;587(11):1617-23. doi: 10.1016/j.febslet.2013.03.042. Epub 2013 Apr 18. 10.1016/j.febslet.2013.03.042 PubMed 23603394